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3’-Seq HSC/MPP1-4

ABSTRACT: Here, we present a 3’-Seq dataset of ex vivo isolated mouse multipotent hematopoietic stem cells (HSC) and multipotent progenitor cells (MPP1-4)

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Pia Somerkamp

PROVIDER: E-MTAB-7391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism.

Sommerkamp Pia P Altamura Sandro S Renders Simon S Narr Andreas A Ladel Luisa L Zeisberger Petra P Eiben Paula Leonie PL Fawaz Malak M Rieger Michael A MA Cabezas-Wallscheid Nina N Trumpp Andreas A

Cell stem cell 20200330 5

Alternative polyadenylation (APA) is emerging as an important regulatory mechanism of RNA and protein isoform expression by controlling 3' untranslated region (3'-UTR) composition. The relevance of APA in stem cell hierarchies remains elusive. Here, we first demonstrate the requirement of the APA regulator Pabpn1 for hematopoietic stem cell (HSC) function. We then determine the genome-wide APA landscape (APAome) of HSCs and progenitors by performing low-input 3' sequencing paired with bioinforma ...[more]

PMID: 32229311

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