Comprehensive RNA-Seq Profiling of the Lung Transcriptome of Bashbay Sheep in Response to Experimental Mycoplasma ovipneumoniae Infection

ABSTRACT: Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characters. It is resistant to Mycoplasma ovipneumoniae (MO) infection, the causative agent of Mycoplasma ovipneumonia, a chronic respiratory disease, which is harmful to the sheep industry. As of date, knowledge regarding the mechanisms responsible for MO pathogenesis in scant. Herein, we report the results of transcriptome profiling of the lung tissue of Bashbay sheep experimentally infected with M. ovipneumoniae strain at 4days post-infection(DPI) and 14DPI in comparison to the mock-infected animals (0d), which was performed by Illumina deep sequencing (RNASeq). The analysis of differentially expressed genes (DEGs) was performed to determine the concomitant gene-specific temporal patterns of mRNA expression in the lung after MO infection. The results showed 1048 DEGs (up-regulated: 575; down-regulated: 473) were screened at 4DPI compared to 0d, and 2823 DEGs (up-regulated: 1362; down-regulated: 1461) were screened at 14DPI compared to 0d. KEGG analysis found that the DEGs at 4DPI and 14DPI compared to 0d were enriched in 245 and 287 pathways respectively, the most closely related to MO infection of which was TLR signaling pathway (p<0.01). Two pathways (First: LAMP-TLR2/TLR6-MyD88-MKK6-AP1-IL1B; Second: LAMP-TLR8- MyD88-IRF5-RANTES) were obtained from the figure of TLR signaling pathway that DEGs related with MO infection were mapped to. GO analysis found that the DEGs in different groups were enriched to 1580 and 4561 terms, the most closely releted to MO infection of which was positive regulation of inflammatory response (p<0.01).Our study shouldpave the road for the further analysis of Mycoplasma ovipneumonia.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Ovis aries

SUBMITTER: Yanming Sun 

PROVIDER: E-MTAB-7408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress