A lower impact of exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment
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ABSTRACT: In the context of tobacco harm-reduction strategy, the potential reduced impact of electronic cigarette (EC) exposure should be evaluated relative to the impact of cigarette smoke (CS) exposure. We conducted a series of in vitro studies to compare the biological impact of an acute exposure to aerosols of “Test Mix” (flavors, nicotine, and humectants), “Base” (nicotine and humectants), and “Carrier” (humectants) formulations using MarkTen® EC device with the impact of CS exposure, at a matching puff number, using human organotypic air–liquid interface buccal and small airway cultures. We measured the concentrations of nicotine and carbonyls deposited in the exposure chamber after each exposure experiment. The deposited nicotine concentrations were used as a measure of nicotine delivery to assess harm reduction relative to CS. The deposited carbonyl concentrations were used as representative measures to assess the reduced exposure to potentially toxic volatile substances. We followed a systems toxicology approach whereby functional biological endpoints, such as histopathology and ciliary beating frequency, were complemented by multiplex and omics assays to measure secreted inflammatory proteins and whole-genome transcriptomes, respectively. Among the endpoints analyzed, the only parameters that showed a significant response to EC exposure were secretion of proteins and whole-genome transcriptomes. Based on the multiplex and omics analyses, the cellular responses to EC aerosol exposure were tissue type-specific; however, those alterations were much smaller than those following CS exposure, even when the EC aerosol exposure under the testing conditions resulted in a deposited nicotine concentration approximately 200 times that in saliva of EC users.
ORGANISM(S): Homo sapiens
SUBMITTER: Alain Sewer
PROVIDER: E-MTAB-7577 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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