Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of Xenopus tRNA isoacceptor families, RNA Polymerase III target genes and selected mRNAs in dorsal embryonic segments of control embryos, or embryos either overexpressing the Polymerase III subunit Polr3G or its paralog Polr3gL at NF stage 25


ABSTRACT: Embryos were injected at the 1 or 2 cell stage with 2ng of Polr3G or Polr3gL mRNA. Embryos were grown alongside batch controls to NF stage 25 and dorsal embryonic segments were dissected to enrich for myogenic tissue. Through inclusion of selected developmental regulatory mRNAs, we aimed to use this data to investigate the relationship between any codon bias in highly expressed mRNAs with expression of their matching tRNA isoacceptors in myogenic lineages and how this changes in response to overexpression of these subunits.

ORGANISM(S): Xenopus tropicalis

SUBMITTER: Caitlin McQueen 

PROVIDER: E-MTAB-7736 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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