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RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

ABSTRACT: More than 5 g/L ethanol was detected at 84 h in the late-exponential phase with 0.1 MPa at pH 6.0 in the batch fermentation of C. ljungdahlii grown autotrophically. Interestingly, ethanol started to be reassimilated in the stationary phase. In order to understand the metabolic flux of ethanol, comparative transcriptome between exponential and stationary phases were performed.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Clostridium ljungdahlii DSM 13528

SUBMITTER: liu ziyong

PROVIDER: E-MTAB-7753 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide.

Liu Zi-Yong ZY Jia De-Chen DC Zhang Kun-Di KD Zhu Hai-Feng HF Zhang Quan Q Jiang Wei-Hong WH Gu Yang Y Li Fu-Li FL

Applied and environmental microbiology 20200702 14

Bioethanol production from syngas using acetogenic bacteria has attracted considerable attention in recent years. However, low ethanol yield is the biggest challenge that prevents the commercialization of syngas fermentation into biofuels using microbial catalysts. The present study demonstrated that ethanol metabolism plays an important role in recycling NADH/NAD<sup>+</sup> during autotrophic growth. Deletion of bifunctional aldehyde/alcohol dehydrogenase (<i>adhE</i>) genes leads to significa ...[more]

PMID: 32414802

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Project description:RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

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