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Unknown,Transcriptomics,Genomics,Proteomics

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ATAC-seq of primary mammalian aortic endothelial cells before and after TNFα stimulation

ABSTRACT: To identify conserved TNFα-induced changes in chromatin-accessibility in mammals, we performed ATAC-seq in primary vascular endothelial cells (ECs) isolated from the aortas of human (HAEC), mouse (MAEC) and cow (BAEC), before and after TNFα. We overlay our data with multi-species NF-κB binding data and identify multiple modes of NF-κB-chromatin interactions that are conserved during mammalian TNFα response. Our cross-species approach identifies conserved changes in chromatin-accessibility at NF-κB binding sites that are disease-relevant and essential during mammalian acute inflammation.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Azad Alizada

PROVIDER: E-MTAB-7878 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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