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Single-cell transcriptional profiling of induced reprogramming trajectories

ABSTRACT: To elucidate the interplay between signal transduction and transcriptional regulation in cellular reprogramming, distinct induction routes from epiblast stem cells to naïve pluripotency were evaluated and compared.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-7901 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Distinct Molecular Trajectories Converge to Induce Naive Pluripotency.

Stuart Hannah T HT Stirparo Giuliano G GG Lohoff Tim T Bates Lawrence E LE Kinoshita Masaki M Lim Chee Y CY Sousa Elsa J EJ Maskalenka Katsiaryna K Radzisheuskaya Aliaksandra A Malcolm Andrew A AA Alves Mariana R P MRP Lloyd Rebecca L RL Nestorowa Sonia S Humphreys Peter P Mansfield William W Reik Wolf W Bertone Paul P Nichols Jennifer J Göttgens Berthold B Silva José C R JCR

Cell stem cell 20190815 3

Understanding how cell identity transitions occur and whether there are multiple paths between the same beginning and end states are questions of wide interest. Here we show that acquisition of naive pluripotency can follow transcriptionally and mechanistically distinct routes. Starting from post-implantation epiblast stem cells (EpiSCs), one route advances through a mesodermal state prior to naive pluripotency induction, whereas another transiently resembles the early inner cell mass and corres ...[more]

PMID: 31422912

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