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RNA-Seq of murine trisomic B cell precursors expressing KRAS_G12D

ABSTRACT: To better understand the molecular bases of the oncogenic cooperation between trisomy 21 and RAS/MAPK pathway activation, KRASG12D was overexpressed in wild-type (WT) or partially trisomic (Ts1Rhr) bone marrow cells. Transduced mCherry-positive murine B cell progenitors were platted in methylcellulose to form B cell precursors colonies (CFU-preB). After a week, B220+/CD19+/CD25- B cells were harvested to perform RNA-sequencing experiments.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Thomas MERCHER

PROVIDER: E-MTAB-8005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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