Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genotype mixing affects gene expression associated with hygienic behavior

ABSTRACT: Analysis used total RNA samples of individual brains of low hygienic strain worker bees that were derived from experimental colonies combined of low hygienic genotypes (L pure colonies) resp. low and high hygienic genotypes (L/H mixed colonies). Four-condition experiment: individuals that did perform hygienic behavior (uncapping) vs individuals that did not perform hygienic behavior (non-hygienic); individuals from L pure colonies vs individuals from L/H mixed colonies. A total of 76 biological replicates was analyzed, subdivided into 24 individuals that did perform hygienic behavior in mixed colonies; 24 individuals that did perform hygienic behavior in pure colonies; 14 individuals that did not perform hygienic behavior in mixed colonies; 14 individuals that did not perform hygienic behavior in pure colonies. Each individual brain RNA sample was divided into two subsamples labeled with cy3 resp. cy5. Loop Design was applied so that each individual brain sample was directly compared to at least two other samples.

ORGANISM(S): Apis mellifera

SUBMITTER: Tanja Gempe

PROVIDER: E-MTAB-801 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mixing of honeybees with different genotypes affects individual worker behavior and transcription of genes in the neuronal substrate.

Gempe Tanja T Stach Silke S Bienefeld Kaspar K Beye Martin M

PloS one 20120214 2

Division of labor in social insects has made the evolution of collective traits possible that cannot be achieved by individuals alone. Differences in behavioral responses produce variation in engagement in behavioral tasks, which as a consequence, generates a division of labor. We still have little understanding of the genetic components influencing these behaviors, although several candidate genomic regions and genes influencing individual behavior have been identified. Here, we report that mix ...[more]

PMID: 22348118

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Project description:As a matter of fact, honeybees are vital for the pollination of more than 80 crops of agricultural interest. However, population decline has become an important global issue causing significant concerns among agricultural experts and the broader public. For this, parasites are known to be the major culprits responsible for the losses of millions of honeybee colonies so far. Among these parasites, Varroa destructor has been identified as a major cause for global losses in Western honeybee (Apis mellifera) colonies. Hygienic behavior (HB), on the other hand, is a collective response by adult honeybees to defend against parasites and diseases that is known to involve in resistance towards Varroosis. Even with the efforts made to elucidate the molecular mechanism underlying HB, it is still not understood. In our study, we have studied the proteomic correlates to HB using a honeybee line (selected for Varroa-specific HB for over a decade in Germany). We sampled individual worker bees from this line that showed HB after closer infrared video observations and compared the proteomes of their mushroom bodies and antennae with those of workers that came from the same set of colonies but didn't show the behaviour. Furthermore, we compared the pupal hemolymph for worker bees of the selected HB line and a control line using state-of-the art techniques of proteomics. We identified a total of 8609 proteins (covered >55% of the honeybee proteome) from these three honeybee tissues. This is the most comprehensive proteomic study of the honeybee HB to date, and the first to focus on individual bees expressing Varroa-specific HB. These results have significantly advanced our knowledge on the biology underlining HB to a new level. The uniquely found functional classes and pathways by the proteins identified in each tissue suggest that hygienic bees have shaped distinct proteome settings to underpin the HB. Moreover, during analysis of pupal hemolymph proteome, the HB-line has adapted a unique strategy to boost an individual and social immunity and drove pupal organogenesis via energy metabolism and protein biosynthesis. Moreover, in the mushroom bodies of different HB phenotypic worker bees, the hygienic bees have enhanced their neuronal sensitivity to promote the execution of HB by activation of synaptic vesicles and calcium channel activities. Moreover, in the antennae of two HB phenotypic worker bees, the hygienic bees have demonstrated strengthening of their sensitivity associated with olfactory senses and signal transmissions, which is important to input a strong signal to the mushroom bodies and initiate HB. In conclusion, our novel findings have significantly extended our understandings of the molecular mechanisms that underline the HB to combat Varroa infestation. Furthermore, we identified a wide array of novel markers that are useful for accelerating marker associated selection of HB to aid in the natural resistance to a parasite blamed for a global decline in honeybee health.

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Dataset Information

Genotype mixing affects gene expression associated with hygienic behavior

Publications

Mixing of honeybees with different genotypes affects individual worker behavior and transcription of genes in the neuronal substrate.

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