Transcription profiling of human testis, primary myeloma cell and normal tissue samples to identify novel cancer-testis genes

ABSTRACT: Cancer-testis (CT) antigens are attractive targets for immunotherapeutic strategies since they are aberrantly expressed in malignant cells and not, or in limited number, in somatic tissues, except germ cells. To identify novel CT genes in multiple myeloma, we used Affymetrix HG-U133 gene expression profiles of 5 testis, 64 primary myeloma cell (MMC) and 24 normal tissue (NT) samples. A 5-filter method was developed to keep known CT genes while deleting non-CT-restricted genes. Starting from 44928 probe sets, including probe sets for 18 known CT genes, we have obtained 82 genes expressed in MMC and testis and not detected in more than 6 NT. This list includes 14 of the 18 known CT genes and 68 novel putative CT genes. Real-time RT-PCR was performed for 34 genes in 12 NT, 3 testis and 5 MMC

ORGANISM(S): Homo sapiens

SUBMITTER: Matthieu Schoenhals 

PROVIDER: E-MTAB-81 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress