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Time course analysis of the cardiac transcriptome in the neonatal period

ABSTRACT: Adult mammalian cardiomyocytes (CM) are differentiated post-mitotic cells that lack significant proliferative potential through their inability to reactivate the cell cycle postnatally. In the week after birth, the mammalian heart goes through distinct stages of cell cycle progression and differentiation that govern the development of the mature adult CM phenotype. By establishing the fundamental framework of the molecular signals governing these early events after birth, a potential treatment strategy that restores the heart’s ability to proliferate, and in theory, undergo ‘repair’ after injury could be developed. At 0 days (d), 1d, 3d, 5d, 7d, 10d, and 15d after birth, hearts from C57BL/6J wild-type mice were excised and processed for total RNA isolation. Samples were analyzed by genome-wide messenger RNA (mRNA) microarray profiling. All experiments were performed on age- and sex-matched mice, with equal ratio of male to female mice.

INSTRUMENT(S): n/a, Affymetrix GeneChip Scanner 3000, Ultra-Turrax, Photometer, Bioanalyzer

ORGANISM(S): Mus musculus

SUBMITTER: Ludger Hauck

PROVIDER: E-MTAB-8168 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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