Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-Seq of mouse Irx3-KO and Control ME3 cells on days 1 and 7 of adipogenic differentiation

ABSTRACT: Irx3 was stably knocked out using a Crispr-Cas9 system. Cells were then input into a differentiation procedure, alongside controls, and RNA was collected for all samples.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Laurence Dyer

PROVIDER: E-MTAB-8209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

The homeobox factor Irx3 maintains adipogenic identity.

Bjune Jan-Inge JI Dyer Laurence L Røsland Gro V GV Tronstad Karl Johan KJ Njølstad Pål R PR Sagen Jørn V JV Dankel Simon N SN Mellgren Gunnar G

Metabolism: clinical and experimental 20191118

<h4>Background</h4>Inhibition of Irx3 and Irx5 has been shown to reduce body weight and white adipose tissue (WAT) mass through cell-autonomous and sympathetic-induced increases in adipocyte beiging and thermogenesis in mice and humans. However, the underlying mechanisms of the Irx control over beiging are still largely unknown, as illustrated by recent reports showing divergent effects of Irx3 on adipocyte metabolism and function. Here, we investigated the role of Irx3 in controlling beige prea ...[more]

PMID: 31751577

Similar Datasets

Project description:The aquatic orthomyxovirus infectious salmon anemia virus (ISAV) is an important pathogen for salmonid aquaculture, however little is known about protective and pathological host responses to infection. We have investigated intracellular responses during cytopathic ISAV infection in the macrophage-like Atlantic salmon kidney (ASK) cell line by microarray analysis (1.8K SFA2.0 immunochip) and a functional assay for glutathione. Gene transcription changed rapidly and consistently with time and with minor differences between two virus isolates. While several pro-inflammatory and antiviral immune genes were induced, genes involved in cell signaling and integrity were down-regulated, suggesting isolation of infected cells from cell-to-cell interaction and responses to external signals. Differential expression of genes regulating cell cycle and apoptosis implied opposite cues from host cell and virus. This was in pace with massive down-regulation of genes involved in biosynthesis and processing of nucleotides and nucleic acids. Significant down-regulation of several genes involved in metabolism of reactive oxygen species suggested increased oxidative stress, which was confirmed by a functional assay showing reduced levels of glutathione during infection. Testing of expression data against a microarray database containing diverse experiments revealed candidate marker genes for ISAV infection. Our findings provide novel insight into cellular host responses and determinants for acute cytopathic ISAV infection. Keywords: Infectious salmon anemia virus (ISAV); Host response; Molecular pathology; Microarray; Macrophage; Oxidative stress ASK cells infected with ISAV isolate 2 and 4 and mock-infected were cultured in triplicates and total RNA pooled from each at the following time-points post-infection; 1, 3 and 5 days. For microarray analysis, single-slide hybridisation was applied between infected RNA (Cy5 labelled) and control RNA (Cy3 labelled) for each time-point. Results were verified by real-time qPCR.

Dataset Information

RNA-Seq of mouse Irx3-KO and Control ME3 cells on days 1 and 7 of adipogenic differentiation

Publications

The homeobox factor Irx3 maintains adipogenic identity.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets