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MiRNA-seq data from healthy and DMD human cells, along skeletal muscle differentiation from induced pluripotent stem cells

ABSTRACT: 3 or 4 healthy cells - 3 DMD cells - 7 time points (tissue-derived myoblasts, tissue-derived myotubes, hiPSCs, Day 3 of differentiation, Day 10 of differentiation, Day 17 of differentiation, Day 25 of differentiation).

INSTRUMENT(S): Ion Torrent Proton

ORGANISM(S): Homo sapiens

SUBMITTER: Virginie Mournetas

PROVIDER: E-MTAB-8293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq data from healthy and DMD human cells, along skeletal muscle differentiation from induced pluripotent stem cells

Project description:3 healthy cells - 3 DMD cells - 7 time points (tissue-derived myoblasts, tissue-derived myotubes, hiPSCs, Day 3 of differentiation, Day 10 of differentiation, Day 17 of differentiation, Day 25 of differentiation) - 1 replicate per cell line for the tissue-derived samples and 3 biological replicates per cell line for the hiPSC-derived samples.

2020-10-05 | E-MTAB-8321 | biostudies-arrayexpress

RNA-seq of Brachypodium distachyon culms from control and BdBAHD01 RNAi plants

Project description:Transcriptome analysis of two independent transgenic lines expressing a construct designed to suppress BdBAHD01. Analysis is intended to quantify suppression, check for off-target effects and look for pleiotropic effects.

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RNA-Seq of Brachypodium distachyon callus treated with methyl-jasmonate

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2017-09-04 | E-MTAB-5413 | biostudies-arrayexpress

RNA-sequencing in HEK293T cells under the knockdown of Arkadia or ESRP2

Project description:We evaluated the role of Arkadia and ESRP2 in HEK293T cells Expression of mRNA in HEK293T cells under the knockdown of Arkadia or ESRP2

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Transcriptomic analysis by AmpliSeq Ion Torrent to explore the effects of the long non-coding RNA TGFB2-AS1 on gene expression in human keratinocytes

Project description:TGFB2-AS1 is a long non-coding RNA which is induced by ΤGFβ signaling. In order to assess the importance of TGFB2-AS1 on the regulation of gene expression, we performed an AmpliSeq transcriptomic array in human keratinocytes (HaCaT), which stably over-express TGFB2-AS1 or control pcDNA3 empty vector. In addition, cells were stimulated with TGFβ1 for 24 hours, in order to observe the effects of TGFB2-AS1 on gene expression, downstream of TGFβ signaling. RNA from the following four conditions was used in this experiment: 1) pcDNA3, 2) pcDNA3+TGFβ1, 3) pcDNA3-TGFB2-AS1, 4) pcDNA3-TGFB2-AS1+TGFβ1. Biological triplicates were used per condition.

2019-07-01 | E-MTAB-7773 | biostudies-arrayexpress

Effect of TTF-1/NKX2-1 expression on TGF-beta induced gene expression in A549 lung cancer cell line.

Project description:TTF-1/NKX2-1 was expressed by adenoviral vector and changes in gene expression were determined by RNA-sequencing. A549 cells were infected with Ad-TTF-1 or Ad-LacZ vectors and stimulated with TGF-beta for 24 hours or left untreated. Expression of polyA RNA was determined.

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2016-06-01 | E-MTAB-4086 | biostudies-arrayexpress

Reduced expression of RBM47 enhances Nrf2 activity to promote tumor growth in A549 lung adenocarcinoma cells

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2016-03-01 | E-GEOD-61910 | biostudies-arrayexpress

RNA-seq of human testicular germ cell cancer

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2019-02-11 | E-MTAB-5746 | biostudies-arrayexpress

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