RNA-seq of human embryonic stem cells during cell cycle progression

ABSTRACT: The experiment was designed to look into transcriptional changes during cell cycle progression in human embryonic stem cells (hESCs). For this, FUCCI hESCs are sorted in Early G1 (EG1), Late G1 (LG1), and S/G2/M phases in triplicates, RNA was extracted and PolyA purified libraries were generated and sequenced in one HIGH Output NextSeq run Cycle, 2 X 75bp, paired-end reads.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Anna Osnato 

PROVIDER: E-MTAB-8347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress