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ScRNAseq of Cyp11a1-expressing cells from mouse melanoma model derived tumors

ABSTRACT: Tumours induce de novo steroidogenesis in immune cells. We wanted to define the gene expression identity of intratumoural steroidogenic immune cells and compare them with the transcriptome with non-steroidogenic cells. Cyp11a1 expression is a faithful biomarker of de novo steroidogenesis (i.e. Cyp11a1+ cells are steroidogenic and Cyp11a1- cells are non-steroidogenic). We inoculated B16-F10 tumor in Cyp11a1-mCherry reporter mice, enriched and purified intratumoral Cyp11a1-mCherry+ and Cyp11a1-mCherry- cells by cell sorting into 96-well plates [with a ratio of 79:15 (mCherry+ : mCherry-) cells per plate] and performed scRNA-seq using SMART-Seq2 platform.

INSTRUMENT(S): BD Influx, Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Krzysztof Polanski

PROVIDER: E-MTAB-8509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tumors induce de novo steroid biosynthesis in T cells to evade immunity.

Mahata Bidesh B Pramanik Jhuma J van der Weyden Louise L Polanski Krzysztof K Kar Gozde G Riedel Angela A Chen Xi X Fonseca Nuno A NA Kundu Kousik K Campos Lia S LS Ryder Edward E Duddy Graham G Walczak Izabela I Okkenhaug Klaus K Adams David J DJ Shields Jacqueline D JD Teichmann Sarah A SA

Nature communications 20200717 1

Tumors subvert immune cell function to evade immune responses, yet the complex mechanisms driving immune evasion remain poorly understood. Here we show that tumors induce de novo steroidogenesis in T lymphocytes to evade anti-tumor immunity. Using a transgenic steroidogenesis-reporter mouse line we identify and characterize de novo steroidogenic immune cells, defining the global gene expression identity of these steroid-producing immune cells and gene regulatory networks by using single-cell tra ...[more]

PMID: 32680985

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