Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of purified cardiomocytes from adult mice (+/- AAV-CCNT1) 4 hours post MycER activation compared controls.

ABSTRACT: Aim: To determine RNA expression levels in heart tissues of mice 4 post Myc activation. Mice were either untreated or systemically infected with a cardiomyocyte specific adeno associated virus (AAV9) at 4 to 6 weeks of age. Cardiomyocytes were isolated from adult mice that were either untreated or infected with AAV9-CCNT1 and were either Myh6-cre;R26CAG-c-MycERT2/+ or R26CAG-c-MycERT2/+ following 4 hours of MycER activation via administration of (Z)-4-hydroxytamoxifen.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Catherine Wilson

PROVIDER: E-MTAB-8515 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of heart from adult mice 4 and 48 hours post MycER activation compared controls

Project description:Aim: To determine RNA expression levels in heart tissues of mice 4 and 48 hours post Myc activation. Mice were systemically infected with a cardiomyocyte specific adeno associated virus (AAV9) at 4 to 6 weeks of age. Hearts were isolated from adult mice that had been infected with AAV9-Ccnt1 and were either Myh6-cre;R26CAG-c-MycERT2/+ or R26CAG-c-MycERT2/+ following 4 hours of MycER activation via administration of (Z)-4-hydroxytamoxifen. Hearts were isolated from adult mice that had been infected with either AAV9-Ccnt1 or AAV9-RFP and were R26CAG-c-MycERT2/+ 48 hours post a single injection of tamoxifen to induce MycER activity.

2020-02-24 | E-MTAB-8462 | biostudies-arrayexpress

RNA-seq of heart tissue from 15 day old mice 4 hours post MycER activation compared to wild type controls. RNA-seq of heart tissues from adult mice that have expressed oncogenic H-Ras for 4 weeks followed by MycER activation for 4 hours compared to MycER negative controls.

Project description:Aim: To determine RNA expression levels in the heart of mice 4 hours post Myc activation. Hearts were isolated from 15 day old wild-type (R26+/+) and R26CAG-c-MycERT2/+ mice following 4 hours of MycER activation via administration of (Z)-4-hydroxytamoxifen. Hearts were isolated from adult TetO-HRas; Myh6-tTA; R26CAG-c-MycERT2/+ and TetO-HRas; Myh6-tTA following 4 weeks of oncogenic H-Ras activation (Doxycycline withdrawal) and following 4 hours of MycER activation via administration of (Z)-4-hydroxytamoxifen.

2020-02-24 | E-MTAB-7595 | biostudies-arrayexpress

RNA-seq of tissue from adult mice 4 hours post MycER activation compared to wild type controls.

Project description:Aim: To determine RNA expression levels in various tissues of mice 4 hours post Myc activation. Tissues were isolated from adult wild-type (R26+/+) and R26CAG-c-MycERT2/+ mice following 4 hours of MycER activation via administration of (Z)-4-hydroxytamoxifen.

2020-02-24 | E-MTAB-7636 | biostudies-arrayexpress

ChIP-sequencing analysis of Myc binding sites 4 hours after Myc activation in liver and heart tissue, from wild type and R26CAG-c-MycERT2 mice

Project description:Aim: To determine Myc binding sites in mouse heart and liver, 4 hours post MycER activation. c-Myc ChIP sequencing performed on chromatin isolated from hearts and livers harvested from wild-type (R26+/+) and R26CAG-c-MycERT2/+ mice 4 hours post administration of (Z)-4-hydroxytamoxifen.

2020-02-24 | E-MTAB-7592 | biostudies-arrayexpress

ChIP-sequencing analysis of RNA Pol II binding sites 4 hours after Myc activation in liver and heart tissue, from wild type and R26CAG-c-MycERT2 mice

Project description:Aim: To determine RNA Pol II binding sites in mouse heart and liver, 4 hours post MycER activation. Pol II ChIP sequencing performed on chromatin isolated from hearts and livers harvested from wild-type (R26+/+) and R26CAG-c-MycERT2/+ mice 4 hours post administration of (Z)-4-hydroxytamoxifen.

2020-03-06 | E-MTAB-7593 | biostudies-arrayexpress

Cardiac-specific YAP activation improve cardiac function and survival in an experimental murine MI model

Project description:In this study, we used a cardiac-specific, inducible expression system to activate YAP in adult mouse heart. Activation of YAP in adult heart promoted cardiomyocyte proliferation and did not deleteriously affect heart function. Furthermore, YAP activation after myocardial infarction (MI) preserved heart function and reduced infarct size. Using adeno-associated virus subtype 9 (AAV9) as a delivery vector, we expressed human YAP in the murine myocardium immediately after MI. We found that AAV9:hYAP significantly improved cardiac function and mouse survival. AAV9:hYAP did not exert its salutary effects by reducing cardiomyocyte apoptosis. Rather, we found that AAV9:hYAP stimulated adult cardiomyocyte proliferation. Gene expression profiling indicated that AAV9:hYAP stimulated cell cycle gene expression, enhanced TGFβ-signaling, and activated of components of the inflammatory response.Cardiac specific YAP activation after MI mitigated myocardial injury after MI, improved cardiac function and mouse survival. These findings suggest that therapeutic activation of hYAP or its downstream targets, potentially through AAV-mediated gene therapy, may be a strategy to improve outcome after MI. Three groups were involved in this study: sham group, AAV9:Luci+MI group and AAV9-YAP+MI group. Each group contained three biological replicates. The sham group had neither myocardial infarction nor AAV injection. The AAV9:Luci +MI(L for brief) group had myocardial infarction and injected with AAV9:Luic. The AAV9:hYAP+MI(YAP for brief) group had myocardial infarction and injected with AAV9:hYAP. 5 days after MI and AAV injection, the heart apexes were collected and the total RNA were isolated for microarray analysis.

2014-05-26 | E-GEOD-54612 | biostudies-arrayexpress

Effect of Fzd9 knock-out on MycER-BclXl insulinoma progression

Project description:Analysis of MycER-BclXl-induced insulinoma in Fzd9 wt and knock out mice after three days of MycER activation Mice (n=4/genotype) were euthanized three days after tamoxifen inoculation and pancreatic islets were isolated and frozen at -80ºC until processing.

2021-02-20 | GSE167073 | GEO

mRNA profiling of injured mouse livers with MycER activation

Project description:This study explore the role of the Myc oncogene in liver injury, in particular for the determination of the cellular programme involved in tissue repair and normalization after injury, a process that is blocked by Myc deregulation. The first objective of this study is to define the transcriptional profile of mouse livers 12 days after injury with or without MycER activation. The second objective is to determine the trancriptionl signature at 1,2,3,7 days after MycER was inactivated in pre injured liver to monitor its normalization.

2022-03-05 | GSE128138 | GEO

Evaluating the effects of cardiac telomerase re-activation after myocardial infarction in adult mice

Project description:Coronary heart disease is a main cause of death in the developed world and treatment success remains modest with high mortality rates within one year after myocardial infarction (MI). Thus, new therapeutic targets and effective treatments are necessary. Short telomeres are risk factors for age-associated diseases including heart disease. Here, we address the potential of telomerase (Tert) activation in prevention of heart failure after MI in adult mice. We use adeno-associated viruses for cardiac-specific Tert expression in a mouse model of MI. We find that upon MI, hearts expressing Tert show attenuated cardiac dilation, improved ventricular function and smaller infarct scars concomitant with increased mouse survival by 17% compared to controls. Furthermore, Tert treatment results in elongated telomeres, increased numbers of Ki67 and pH3-positive cardiomyocytes and a gene expression switch towards a regeneration signature of neonatal mice. Our work highlights telomerase activation as a novel therapeutic strategy to prevent heart failure after MI. Mice of one year of age were left untreated (control) or injected with 5*10^11 adeno associated viruses particles of serotype 9 (AAV9) that carry either en empty expression cassette or express telomerase under control of the CMV promoter. Virus injected mice then underwent myocardial infarction induced through permenant left anterior descending artery (LAD) ligation. Mice that survived for six weeks after LAD ligation were sacrificed and 4 hearts per group (AAV9-empty or AAV9-Tert) and 3 control hearts (no virus treatment, no ligation) were subjected to total RNA isolation for micro array analysis.

2014-12-15 | E-GEOD-62973 | biostudies-arrayexpress

BVES-HA IP-mass from AAV9-BVES-HA injected mouse skeletal muscle

Project description:To identify BVES-interacting proteins from mouse skeletal muscle, we performed IP-mass from AAV9-BVES-HA injected mouse skeletal muscle using the anti-HA magnetic beads.

2023-02-27 | PXD036346 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data