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Expression analysis in brain endothelial cells of Gαq/11beKO mice to elucidate CO2-induced vasoreactivity mechanism

ABSTRACT: CO2-induced vasoreactivity is an important mechanism to remove CO2 from the brain. Our previous work has shown that in brain endothelial cells Gαq and Gα11, that can replace each other and induce a common intracellular pathway, are required for normal vascular reactivity to CO2 stimulation. Additionally, we found different effects on vascular diameter dependent on the brain area. In the cortex, vessel dilate as response to CO2 stimulation, in the retrotrapezoid nucleus (RTN) of the brainstem, vessels constrict upon CO2 stimulation. To examine the potential basis for the opposing effects of CO2 on the vascular reactivity in brainstem compared to other brain areas, we prepared cortical and brainstem vessel fragments of control and Gαq/11beKO mice and performed microarrays to determine mRNA expression.

ORGANISM(S): Mus musculus

SUBMITTER: Oliver Hummel

PROVIDER: E-MTAB-8521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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