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Liquid Biopsy-Based Single-Cell Transcriptome Profiling Characterizes Heterogeneity of Disseminated Tumor Cells from Lung Adenocarcinoma

ABSTRACT: To decipher the heterogeneity and distinct molecular signatures of disseminated tumor cells, we profiled the transcriptomes single cells from malignant pleural effusion. RNA-seq was done using the standard 10x chromium v3 chemistry.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Yuliang Deng

PROVIDER: E-MTAB-8530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Liquid Biopsy Based Single-Cell Transcriptome Profiling Characterizes Heterogeneity of Disseminated Tumor Cells from Lung Adenocarcinoma.

Dong Yu Y Wang Zhuo Z Shi Qihui Q

Proteomics 20200131 13

The advent of rapid and inexpensive sequencing technology allows scientists to decipher intra-tumor heterogeneity spatially and temporally for resolving the evolutionary history of tumor and the underlying mechanism. However, studies on characterizing heterogeneity of disseminated tumor cells (DTCs) in liquid biopsies are rare because of the rarity and low viability of DTCs as well as a large number of non-tumor cells. Here, high-throughput single-cell transcriptome sequencing technology and rar ...[more]

PMID: 31960581

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