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Transcription start site identification in Bacillus subtilis

ABSTRACT: Cappable-seq was used to map transcription start sites globally in wild type Bacillus subtilis. Total RNA was isolated from cells grown in LB media until exponential phase. RNA corresponding to transcription start sites was capped with a 5' biotin tag, which was used for enrichment via a pull down with streptavidin beads. Enriched RNA was converted to cDNA and then subjected to illumina sequencing.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: David Forrest

PROVIDER: E-MTAB-8582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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