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RNA-seq study of the response of the diatom Leptocylindrus aporus to changing temperature conditions.

ABSTRACT: The goal of the experiment was to compare the behavior and responses of a warm water diatom species in three different temperatures, low, intermediate and high, with the final aim of investigating possible intraspecific variability and better understanding the physiological plasticity and/or adaptation of diatoms to a wide range of conditions. Three different strains were used as replicates, B651,1A1,3A6. They were isolated from sea surface waters at the LTER-MC site (40°48.5’N, 14°15’E) in the Gulf of Naples on 21/08/2010, 20/12/2013 and 28/01/2014 respectively and grown in cultures. All three strains were acclimated to 13°C, 19°C and 26°C at a light intensity of 100 μmol photons m-2 sec-1 and a photoperiod of L:D, 12:12 and grown to a final concentration of 50,000 cells/ml ensuring they were still at exponential phase.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Leptocylindrus aporus

SUBMITTER: Mariella Ferrante

PROVIDER: E-MTAB-8596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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