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Time-course transcriptome analysis of mature nodules in Medicago truncatula grown in split-root under different nitrogen (N) supply

ABSTRACT: The experiment aim is the identification of the transcriptional response of mature nodules of Medicago truncatula inoculated with the strain MD4 of Sinorhizobium medicae in response to different nitrogen (N) supply (N-satiety and N-deficient conditions). We performed a time-course (1 and 3 days treatment) split-root experiment to identify plant systemic responses: the root systems of nodulated symbiotic plants were divided into two compartments, one of them treated and the other kept on control conditions. Nodules were isolated from the non-treated side of the split root and total RNA isolated for RNAseq analysis.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Medicago truncatula

SUBMITTER: Ilana Lambert

PROVIDER: E-MTAB-8597 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Responses of mature symbiotic nodules to the whole-plant systemic nitrogen signaling.

Lambert Ilana I Pervent Marjorie M Le Queré Antoine A Clément Gilles G Tauzin Marc M Severac Dany D Benezech Claire C Tillard Pascal P Martin-Magniette Marie-Laure ML Colella Stefano S Lepetit Marc M

Journal of experimental botany 20200801 16

In symbiotic root nodules of legumes, terminally differentiated rhizobia fix atmospheric N2 producing an NH4+ influx that is assimilated by the plant. The plant, in return, provides photosynthates that fuel the symbiotic nitrogen acquisition. Mechanisms responsible for the adjustment of the symbiotic capacity to the plant N demand remain poorly understood. We have investigated the role of systemic signaling of whole-plant N demand on the mature N2-fixing nodules of the model symbiotic associatio ...[more]

PMID: 32386062

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