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Single cell RNA-seq of 3 key time points in oesophageal post-natal development

ABSTRACT: The purpose of this single cell experiment is to compare and characterize at molecular level oesophageal epithelial cells at different points in post-natal development (post-natal day 7 and 28) and at the onset of homeostasis (over p70). With the aim of identifying differences in timepoints showing the key molecular markers of the transition from a rapidly expanding tissue to one that is stable in size and function. Previous data has suggested this transition point occurs around P28 and we hope to see here a similarity in the profile of P28 samples with that of adults. Single cell suspensions of pooled oesophageal epithelial and stroma cells were sorted for EpCam+ and CD45- expression to select for epithelium cells for RNA-sequencing.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Maria Alcolea

PROVIDER: E-MTAB-8662 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A biomechanical switch regulates the transition towards homeostasis in oesophageal epithelium.

McGinn Jamie J Hallou Adrien A Han Seungmin S Krizic Kata K Ulyanchenko Svetlana S Iglesias-Bartolome Ramiro R England Frances J FJ Verstreken Christophe C Chalut Kevin J KJ Jensen Kim B KB Simons Benjamin D BD Alcolea Maria P MP

Nature cell biology 20210510 5

Epithelial cells rapidly adapt their behaviour in response to increasing tissue demands. However, the processes that finely control these cell decisions remain largely unknown. The postnatal period covering the transition between early tissue expansion and the establishment of adult homeostasis provides a convenient model with which to explore this question. Here, we demonstrate that the onset of homeostasis in the epithelium of the mouse oesophagus is guided by the progressive build-up of mecha ...[more]

PMID: 33972733

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