Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

41 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Populus × canescens roots and leaves in reaction to fungal (Laccaria bicolor) volatiles

ABSTRACT: Populus × canescens plantlets were grown in Petri dishes on MS medium. 14 days old plantlets were co-incubated with L. bicolor without physical contact. After 2 and 7 days of co-incubation, roots and leaves were harvested.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Populus tremula x Populus alba

SUBMITTER: Mareike Jakobi

PROVIDER: E-MTAB-8683 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Arabidopsis thaliana root parts in reaction to fungal (Laccaria bicolor) volatiles

Project description:Arabidopsis thaliana plants were grown from seeds in Petri dishes on MS medium. 4 days old plants were co-incubated with L. bicolor without physical contact. After 2 days of co-incubation, roots were either harvested whole or separated into the root tip (ca. 1/4 of the whole root) and the lateral root zone (= remaining root).

2020-12-10 | E-MTAB-8682 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana roots and leaves in reaction to fungal (Laccaria bicolor) volatiles

Project description:Arabidopsis thaliana plants were grown from seeds in Petri dishes on MS medium. 4 days old plants were co-incubated with L. bicolor without physical contact. Roots were harvested after 0, 2, 4 and 7 days of co-incubation, rosette leaves were harvested after 4 days of co-incubation.

2020-12-10 | E-MTAB-8684 | biostudies-arrayexpress

Ethylene and jasmonic acid act as negative modulators during mutualistic symbiosis between Laccaria bicolor and Populus roots

Project description:The Poplar transcriptome was analyzed in mycorrhizal root tips in contact with Laccaria bicolor for 2 weeks. During mycorrhization the roots were treated with either 250M-BM-5m ACC, 10nM JA or 500M-BM-5M SA and compared to untreated mycorrhiza or control roots without contact to L. bicolor. In addition the poplar mutants 35S::PttACO1 and 35S::Atetr1 were used We performed 27 hybridizations (NimbleGen) with samples derived from Populus tremula x Populus alba clone 717-1B4 control roots, untreated mycorrhiza, SA-treated mycorrhiza, ACC-treated mycorrhiza and JA-treated mycorrhiza (3 biological replicates each) as well as Populus tremula x Populus tremuloides T89 control roots, mycorrhiza, 35S::PttACO1 mycorrhiza and 35S::Atetr1-1 mycorrhiza (3 biological replicates). All samples were labeled with Cy3.

2013-12-19 | E-GEOD-53475 | biostudies-arrayexpress

Gene expression of Laccaria bicolor S238N free-living mycelium grown on different media

Project description:Transcript profiles of Laccaria bicolor S238N mycelium on various media were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) Laccaria bicolor genome sequence version 1. One goal was to evaluate the effect of nutrient deprivation on the transcriptome of Laccaria bicolor. We performed 15 hybridizations (Roche-NimbleGen) with samples derived from Laccaria bicolor free-living mycelium grown on MMN medium, on MMN with a 10 times reduction in all major macro-elements, on MMN with a 10 times reduction in the quantity of glucose or onto agar medium supplemented with the same nutrients used to fertilize our mycorrhization experiments.

2012-03-01 | E-GEOD-35556 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana leaves as reaction to fungi (Laccaria bicolor) infection of the roots

Project description:Arabidopsis thaliana plants were grown from seeds. Roots of 7 day old plants were inoculated with L. bicolor. After 2 days, leaves were harvested, shock frozen in liquid nitrogen and prepared for RNA extraction

2020-06-30 | E-MTAB-8544 | biostudies-arrayexpress

Effect of Laccaria bicolor, MiSSP7 from L.bicolor and Jasmonic acid on the Poplar transcriptome

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-04-17 | E-GEOD-56865 | biostudies-arrayexpress

Laccaria-Douglas fir: A Timecourse of Mycorrhizal Root Tip Development

Project description:This study characterizes the transcriptomic alterations of Laccaria bicolor S238N during interaction with Pseudotsuga menziesii. Three time-points were analyzed, two weeks, four weeks and six weeks after inoculation and compared to the transcriptome of free-living mycelium from Laccaria bicolor S238N. We performed 9 hybridizations (NimbleGen) with samples derived from Pseudotsuga menziesii /L.bicolor mycorrhizal root tips. Samples were taken after 2,4 and 6 weeks of interaction (three biological replicates). These samples were compared to free-living mycelium from Laccaria bicolor S238N (three biological replicates). All samples were labeled with Cy3.

2014-10-10 | E-GEOD-62226 | biostudies-arrayexpress

Effects of Laccaria bicolor treatment on Populus × canescens leaf transcriptome

Project description:The roots of four week old Populus × canescens were treated with Laccaria bicolor for one, two, four and seven days. Leaves three, four and five were harvested, pooled and used for RNA extraction and sequencing. The RNA of three biological samples was pooled after extraction. The experiment was repeated three times.

2024-05-01 | E-MTAB-12654 | biostudies-arrayexpress

Laccaria-Poplar: A Timecourse of Mycorrhizal Root Tip Development

Project description:This study characterizes the transcriptomic alterations of Laccaria bicolor S238N during interaction with P. trichocarpa. Four time-points were analyzed, two weeks, four weeks , six weeks and twelve weeks after inoculation and compared to the transcriptome of free-living mycelium from Laccaria bicolor S238N We performed 16 hybridizations (NimbleGen) with samples derived from P.trichocarpa/L.bicolor mycorrhizal root tips. Samples were taken after 2,4,6 and 12 weeks of interaction (four biological replicates). These samples were compared to free-living mycelium from Laccaria bicolor S238N (three biological replicates). All samples were labeled with Cy3.

2014-10-10 | E-GEOD-62225 | biostudies-arrayexpress

Transcription profiling of Populus trichocarpa roots in response to species diversity levels

Project description:Changes in gene expression in P. trichocarpa leaves was measured in response to growth competition by P. x canescens.Square petri dishes (12 x 12 cm) were filled autoclaved sand, 3 plantlets and Woody Plant Medium (WPM). In each petri dish we are growing 3 plantlets. As control 3 plantlets of P. trichocarpa and for the experiment a mixture of P. canescens and P. trichocarpa were P. trichocarpa is all the time the middle plantlet.

2015-05-22 | E-MEXP-3909 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data