Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Two colour microarray of Saccharomyces cerevisiae cultivation and bioethanol production with aeration using microbubbles or regular bubbles

ABSTRACT: Microbubbles are micron-sized bubbles generated using a fluidic oscillator. They have the potential to decrease the cost of biotechnology processes through decreasing the energy required for aeration and mixing. However, little is known about microbial physiology when grown on microbubbles. This project compared Saccharomyces Cerevisiae growth and transcriptomic effects when cells were grown with microbubble or regular bubble aeration

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Joseph Webb

PROVIDER: E-MTAB-8696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A microbubble-sparged yeast propagation-fermentation process for bioethanol production.

Raghavendran Vijayendran V Webb Joseph P JP Cartron Michaël L ML Springthorpe Vicki V Larson Tony R TR Hines Michael M Mohammed Hamza H Zimmerman William B WB Poole Robert K RK Green Jeffrey J

Biotechnology for biofuels 20200608

<h4>Background</h4>Industrial biotechnology will play an increasing role in creating a more sustainable global economy. For conventional aerobic bioprocesses supplying O<sub>2</sub> can account for 15% of total production costs. Microbubbles (MBs) are micron-sized bubbles that are widely used in industry and medical imaging. Using a fluidic oscillator to generate energy-efficient MBs has the potential to decrease the costs associated with aeration. However, little is understood about the effect ...[more]

PMID: 32523617

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Project description:We have used human umbilical vein endothelial cell line (HUVEC) as a model to investigate the effect of ultrasound (US) activated micro-bubbles on sensitizing the tumour response to radiation and to check the implication of this approach on gene expression. The use of micro-bubbles in cancer therapy is a novel approach that is being recently investigated, and patterns of gene expressions were not yet characterized. Gene analysis has identified about 19,264 genes; where, 239 – 517 genes have shown more than two folds up-regulation in response to the different treatments. This included a number of genes that are known to be involved in apoptosis and ceramide-induced apoptosis pathways, such as Sphingomyelin phosphodiesterase 2, neutral sphingomyelinase (SMPD2), sphingomyelin phosphodiesterase 1, acid lysosomal (ASM; SMPD1), UDP-galactose ceramide galactosyltransferase (UGT8), cytochrome c oxidase (COX6B1), Caspase-9 and mitogen-activated protein kinase kinase 1 (MAP2K1). To verify these results, we have performed Real time RT-PCR, which indicated an up-regulation that ranged from 2 to 200 folds, specifically in the combined treatment of US, 3.3% micro-bubbles and 8 Gy. The observed higher levels of expression of UGT8, SMPD2 and Caspase 9-alpha when compared to the control or to radiation only indicated the involvement of ceramide, which possibly induces apoptosis . These results support the hypothesis that micro-bubbles play a role in increasing sensitization to radiation, and can prove to be an effective cancer therapeutic approach. Gene analysis has identified about 19,264 genes; where, 239 – 517 genes have shown more than two folds up-regulation in response to the different treatments. This included a number of genes that are known to be involved in apoptosis and ceramide-induced apoptosis pathways, such as Sphingomyelin phosphodiesterase 2, neutral sphingomyelinase (SMPD2), sphingomyelin phosphodiesterase 1, acid lysosomal (ASM; SMPD1), UDP-galactose ceramide galactosyltransferase (UGT8), cytochrome c oxidase (COX6B1), Caspase-9 and mitogen-activated protein kinase kinase 1 (MAP2K1). To verify these results, we have performed Real time RT-PCR, which indicated an up-regulation that ranged from 2 to 200 folds, specifically in the combined treatment of US, 3.3% micro-bubbles and 8 Gy. The observed higher levels of expression of UGT8, SMPD2 and Caspase 9-alpha when compared to the control or to radiation only indicated the involvement of ceramide, which possibly induces apoptosis . These results support the hypothesis that micro-bubbles play a role in increasing sensitization to radiation, and can prove to be an effective cancer therapeutic approach.

Dataset Information

Two colour microarray of Saccharomyces cerevisiae cultivation and bioethanol production with aeration using microbubbles or regular bubbles

Publications

A microbubble-sparged yeast propagation-fermentation process for bioethanol production.

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