Unknown,Transcriptomics,Genomics,Proteomics

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Elucidating the role of polygalacturonase genes in strawberry fruit softening


ABSTRACT: The aim of this study was to determine the role of genes encoding polygalacturonases in strawberry fruit softening. To this purpose, several transgenic lines, cv. Chandler, were generated: plants with PG genes FaPG1 or FaPG2 downregulated, alone or in combination, by antisense transformation. Plants were grown in a confined greenhouse and fruits were harvested at the stage of full ripeness (100% of fruit surface red). The results obtained indicate that the silencing of these genes reduced fruit softening at similar level but there is not a sinergistic effect on fruit firmness.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Fragaria x ananassa

SUBMITTER: Antonio Matas 

PROVIDER: E-MTAB-8736 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications


To disentangle the role of polygalacturonase (PG) genes in strawberry softening, the two PG genes most expressed in ripe receptacles, FaPG1 and FaPG2, were down-regulated. Transgenic ripe fruits were firmer than those of the wild type when PG genes were silenced individually. Simultaneous silencing of both PG genes by transgene stacking did not result in an additional increase in firmness. Cell walls from ripe fruits were characterized by a carbohydrate microarray. Higher signals of homogalactur  ...[more]

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