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Transcription profiling by array of cancer associated fibroblasts from pancreatic ductal adenocarcinoma in comparison to normal dermal fibroblasts

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive tumor type with extremely high mortality (up to 92%) during 5 years after diagnosis. Here, cancer associated fibroblasts (CAF) from PDAC were compared to CAF from melanoma metastases (MELF) and to normal dermal fibroblasts (DF). The analysis was performed in three biological replicates for normal fibroblasts, eight biological replicates for PDAC CAF, and four biological replicates for melanoma CAF. Further technical replicates were used to improve data quality.

ORGANISM(S): Homo sapiens

SUBMITTER: Hynek Strnad

PROVIDER: E-MTAB-8764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts.

Novák Štepán Š Kolář Michal M Szabó Arpád A Vernerová Zdena Z Lacina Lukáš L Strnad Hynek H Šáchová Jana J Hradilová Miluše M Havránek Jan J Španko Michal M Čoma Matúš M Urban Lukáš L Kaňuchová Miriam M Melegová Nikola N Gürlich Robert R Dvořák Josef J Smetana Karel K Gál Peter P Szabo Pavol P

Cancer genomics & proteomics 20210501 3

<h4>Background/aim</h4>Pancreatic ductal adenocarcinoma (PDAC) still represents one of the most aggressive cancers. Understanding of the epithelial-mesenchymal crosstalk as a crucial part of the tumor microenvironment should pave the way for therapies to improve patient survival rates. Well-established cell lines present a useful and reproducible model to study PDAC biology. However, the tumor-stromal interactions between cancer cells and cancer-associated fibroblasts (CAFs) are still poorly und ...[more]

PMID: 33893076

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