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ChIP-seq analysis of Escherichia coli sigma 38 binding in cells lacking H-NS

ABSTRACT: The experiment contains ChIP-seq data for Escherichia coli strain RPB104 hns::kan. The strain was grown at 37 degrees in LB medium to stationary phase and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Escherichia coli K-12

SUBMITTER: David Grainger

PROVIDER: E-MTAB-8778 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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