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Transcriptome profiling using microarrays of four human metformin-treated non-small cell lung cancer cell-lines

ABSTRACT: To examine the effect of metformin on lung cancer biology, human lung A549 adenocarcinoma, H460 large cell carcinoma, and H226 and H1299 squamous cell carcinoma cell-lines were grown in RPMI-1640 medium with 10% v/v fetal bovine serum and with or without 15 uM metformin hydrochloride for 7-8 days. Medium (with any metformin) was replaced every two days. Paired cultures with and without metformin were grown and maintained in parallel. Three separate paired cultures, all seeded with same stock of frozen cells, were grown. Cells, within 15%-95% confluence range, were harvested by scraping. Total RNA from cells was prepared using Norgen Biotek® Total RNA Isolation kit (with on-column DNAse I treatment). All RNA integrity number (RIN) values were greater than 8.2.

ORGANISM(S): Homo sapiens

SUBMITTER: Santosh Patnaik

PROVIDER: E-MTAB-8781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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