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RIL-seq of EPEC E2348/69 hfq-flag, in virulence activating and non-activating conditions.

ABSTRACT: RIL-seq experiment of EPEC hfq-flag mutant, in activating- conditions:growth on DMEM at 37°C to mid exponential growth phase (e.g., OD600=0.3). In these conditions EPEC strongly expresses its major virulence components, T3SS and BFP, mimicking infection. Non-activating conditions: overnight growth of static culture on LB medium at 37°C where virulence factors are not expressed. RIL-seq experiments are designed to reveal the interactions of sRNA and their targets.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Escherichia coli

SUBMITTER: Sivan Pearl

PROVIDER: E-MTAB-8806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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