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RNA-seq on Hu5/KD3 cells cultured with or without DAPT at proliferating stage

ABSTRACT: DAPT(a Notch inhibitor) promted myotube formation of human muscle progenitors. To clarify the Notch signal-responsive genes that inhibit or augment myotube formation, we examined the gene expression in Hu5/KD3 (immortalized human myoblast) treated with DAPT for three days by RNA-seq analysis. Hu5/KD3 cells were seeded 5×104 cells per collagenⅠcoated 24-well plate, cultured with DMEM (High Gluc.) containing 20% FBS, 2% UltroserG and supplemented with penicillin(100 units/mL), streptomycin (100 units/mL), Amphotericin B (250 ng/mL), L-Glu (2 mmol/L). Next day, started to treat with 0.1% DMSO or 10 μM DAPT in 10% FBS/DMEM. After three days treatment, total RNA was extracted as one sample from three wells by using RNeasy mini kit (QIAGEN). RNA libraries were prepared for sequencing using standard Illumina protocols

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Ken'ichiro Nogami

PROVIDER: E-MTAB-8825 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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