Unknown,Transcriptomics,Genomics,Proteomics

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A natural variant of the sole pyruvate kinase of fission yeast lowers glycolytic flux triggering increased respiration and oxidative-stress resistance but decreased growth


ABSTRACT: To investigate the downstream effects of a polymorphism in the pyk1 gene of Schizosaccharomyces pombe (A343T) we generated a strain that differed from the lab strain (JB50) only in the amino acid at position 343 of pyk1 where a threonine was substituted with an alanine. Five replicates were grown for each strain and harvested in log phase. From each of these cultures RNA was isolated and underwent polyA-enrichment. Reads were sequenced and mapped against the strain-specific genomes.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Jan Grossbach 

PROVIDER: E-MTAB-8847 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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