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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic analysis of osteoblastic cells derived from human mesenchymal stem cells, incubated with secreted factors release by adipocytes against non-incubated controls

ABSTRACT: Our preliminary findings lead us to propose bone marrow adipocyte secretions as new actors of bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocyte-like phenotype. To further decipher the molecular changes triggered by the coculture, we performed transcriptional analyses using Agilent 60-mer Sure Print G3 Human Gene Expression Microarray Kit containing a 50, 599 probes. Transcriptional changes were monitored in 7 biological replicates each consisting of osteoblasts grown alone (OB) and cocultured osteoblasts (OB-CC) upon 9h or 48h and adipocytes (AD) at day 14 of differentiation placed in serum-free medium for 9h or 48h.

ORGANISM(S): Homo sapiens

SUBMITTER: ALINE CLABAUT

PROVIDER: E-MTAB-8849 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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