Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Populus euphratica roots and leaves in response to 150 mM NaCl

ABSTRACT: Wild type Populus euphratica were multiplied by in vitro micropropagation and grown in aerated hydroponics using Long-Ashton nutrient solution. Plants were adapted to 150 mM NaCl by increasing the concentration in the nutrient solution over several days. Root and leaf samples were harvested after growing in 150 mM NaCl for 2 and 12 days.

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Populus euphratica

SUBMITTER: Shayla Sharmin

PROVIDER: E-MTAB-8988 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:affy_nitrogen_medicago - affy_nitrogen_medicago - Experiment has been designed to characterize the molecular expression patterns associated to a contrasted modification of the nitrogen status of the whole plant. The systemic effects of nitrogen status modifications are investigated and compared on non nodulated plant supplied with NO3, NH4 or nodulated plants (Sinorhizobium meliloti 2011) supplied with air. The root systems were separated in two compartments of unequal sizes (split root system). Two treatments were applied on the larger compartment in order to modulate the nitrogen status of the plant: for the S treatment, roots are supplied with nutrient solution containing 10 mM NH4NO3,, whereas for the C treatment, roots are supplied with nitrogen free medium. In the case of N2 fixing plants, N limitation was obtained by replacing air by a mixture of Ar and O2 80 per cent and 20 per cent. The effects of these treatments were investigated on roots of the minor compartment supplied continuously with either NO3 1 mM, NH4 1 mM or air (N2) and on the shoots. We were also interested in the molecular expression patterns associated to the roots deprived of N.-The root system of non-nodulated (NO3- and NH4+) or nodulated (N2) plants is split into two unequal parts and each one is installed in a separate compartment. For the S treatement, the major root part is supplied with NH4NO3 10 mM whereas the minor part is supplied with either NO3- 1mM, NH4+ 1mM or N2. For the C treatement, the major root part is supplied with nitrogen-free nutrient solution whereas the minor part is supplied with either NO3- 1mM, NH4plus 1mM or N2. Each treatement is four days long. Samples of roots of six biological types (NO3S, NO3C, NH4S, NH4C, N2S and N2C) were collected. Two biological repeats per biological types have been analyzed. The effect of the S and C treatments were investigated for each N sources by comparing Affymetrix transcriptomes (NO3C vs NO3S, NH4C vs NH4S, N2C vs N2S). Experiment Overall Design: 26 arrays - medicago

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Transcription profiling of Populus euphratica roots and leaves in response to 150 mM NaCl

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