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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of siRNA transfected HEK293T cells untreated or treated with TNF-alpha to study the effect of siRNA-mediated knockdown of different genes

ABSTRACT: HEK293T cells were treated with TNF-alpha for different periods of time to study the effect of siRNA-mediated knockdown of different genes (negative control: Renilla luciferase; positive controls: TNFRSF1A, RelA; gene of interest: CASP4) on TNF-induced gene expression

ORGANISM(S): Homo sapiens

SUBMITTER: Dorothee Nickles

PROVIDER: E-MTAB-900 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A genome-wide RNA interference screen identifies caspase 4 as a factor required for tumor necrosis factor alpha signaling.

Nickles Dorothee D Falschlehner Christina C Metzig Marie M Boutros Michael M

Molecular and cellular biology 20120625 17

Tumor necrosis factor alpha (TNF-α) is a potent inflammatory cytokine secreted upon cellular stress as well as immunological stimuli and is implicated in the pathology of inflammatory diseases and cancer. The therapeutic potential of modifying TNF-α pathway activity has been realized in several diseases, and antagonists of TNF-α have reached clinical applications. While much progress in the understanding of signaling downstream of the TNF-α receptor complex has been made, the compendium of facto ...[more]

PMID: 22733992

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