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Integrative single-cell RNA-Seq and ATAC-Seq analysis of human foetal liver and bone marrow haematopoiesis (scRNA-Seq)

ABSTRACT: This is plate-based (SmartSeq2-Seq) single-cell transcriptome data set from 15 human foetal livers and bone marrow (17-22 PCW). It includes 5,867 cells with an average of 3,074 genes per cell before QC and 4,504 cells with an average of 3,642 genes per cell after QC.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Simone Riva

PROVIDER: E-MTAB-9067 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis.

Ranzoni Anna Maria AM Tangherloni Andrea A Berest Ivan I Riva Simone Giovanni SG Myers Brynelle B Strzelecka Paulina M PM Xu Jiarui J Panada Elisa E Mohorianu Irina I Zaugg Judith B JB Cvejic Ana A

Cell stem cell 20201221 3

Regulation of hematopoiesis during human development remains poorly defined. Here we applied single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to over 8,000 human immunophenotypic blood cells from fetal liver and bone marrow. We inferred their differentiation trajectory and identified three highly proliferative oligopotent progenitor populations downstream of hematopoietic stem cells (HSCs)/multipotent progenitors (MPPs). Al ...[more]

PMID: 33352111

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Integrative single-cell RNA-Seq and ATAC-Seq analysis of human foetal liver and bone marrow haematopoiesis (scATAC-Seq)

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