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Analysis of the transcriptional response to bleomycin-induced DNA damage in Physcomitrella patens wild type and mutants defective in the ATM, ATR genes and the double mutant

ABSTRACT: To analyse the early phase of the transcriptional response to bleomycin-induced damage and its dependence on the severity of the damage, we used exposure for 1 hour to a sub-lethal (0.3 u/l) and a lethal (3 u/l) dose of bleomycin. In addition, an aliquot of the 0.3 u/l treatment was washed free of bleomycin and incubated for an additional 3 hours in its absence. Thereafter total RNA was prepared and transcriptional patterns were determined by SuperSAGE (Molina et al., 2008, BMC genomics 9, 553 and Matsumura et al., 2010, PLOS ONE 5, e12010. The transcriptional response to bleomycin in wild-type and the Ppatm-1, Ppatr-1 and Ppatmatr-1 mutants was analysed as above in a second, separate experiment using the 1 hour pulse treatment with a sub-lethal (0.3 u/l) concentration followed by the 3 hour recovery period .

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Physcomitrella patens

SUBMITTER: Ralf Horres

PROVIDER: E-MTAB-9082 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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