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RNAseq of ZC3H5 interacting mRNA

ABSTRACT: Bloodstream-form trypanosomes (Lister 427) constitutively expressing ZC3H5-TAP (Tb927.3.740) were used. The protein was pulled down with an IgG column , then the protein and bound RNA was eluted using TEV protease. RNA was then sequenced from unbound (flow-through) and bound (eluate) fractions.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Trypanosoma brucei brucei TREU927

SUBMITTER: Christine Clayton

PROVIDER: E-MTAB-9084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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