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MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping

ABSTRACT: Genotyping of RpoD mutants via amplicon sequencing from the following manuscript: \\"Systematic dissection of σ70 sequence diversity and function in bacteria\\" by Park and Wang (2020). Includes raw sequencing reads from samples from MAGE-seq single codon saturation mutagenesis and high-throughput fitness competition experiment as well as the RpoD ortholog mutants generated through recombineering and CRISPR selection.

INSTRUMENT(S): Illumina MiSeq, NextSeq 550

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: jimin park

PROVIDER: E-MTAB-9103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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