Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Single cell transcriptomics profiling of vessel co-option


ABSTRACT: Vessel co-option is an alternative mode of tumor vascularization, which contributes to resistance to anti-angiogenic therapy (AAT). In contrast to vessel sprouting (angiogenesis), knowledge about the mechanisms underlying vessel co-option is minimal, precluding therapeutic strategies. We therefore single-cell RNA-sequenced 31,964 cells from a murine lung metastasis model with vessel co-option, characterized by resistance to AAT. Unexpectedly, co-opted endothelial cells (ECs) were transcriptomically indistinguishable from healthy ECs and lacked an activation signature, while co-opted pericytes expressed a quiescence signature, in contrast to activated pericytes during angiogenesis. Compared with cancer cells during angiogenesis, co-opting cancer cells were phenotypically more diverse and enriched in invasive subpopulations. Together, these data reveal new insight into vessel co-option, with possible implications for the development of therapeutic targets.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Shawez Khan 

PROVIDER: E-MTAB-9227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2020-05-20 | E-MTAB-7458 | biostudies-arrayexpress
2020-02-13 | E-MTAB-8077 | biostudies-arrayexpress
2020-03-07 | E-MTAB-8031 | biostudies-arrayexpress
| PRJEB39195 | ENA
2019-12-30 | E-MTAB-6308 | biostudies-arrayexpress
2020-06-25 | E-MTAB-8119 | biostudies-arrayexpress
2023-01-05 | E-MTAB-12022 | biostudies-arrayexpress
2015-01-01 | E-GEOD-61582 | biostudies-arrayexpress
2024-01-11 | PXD042346 | Pride
2019-12-12 | E-MTAB-8145 | biostudies-arrayexpress