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Histone acetylation H3K9ac ChIP-seq of Arabidopsis thaliana leaves for three genotypes (Col-0 wild type, gsnor1-3, axe1-5/hda6) in low light and dark conditions

ABSTRACT: Plants were grown for 4 weeks under short day (10 h light/14 h dark and 20 °C/16 °C, respectively). The relative humidity during the day and night was 50 %. Then, plants were transferred at 11 am (5 h after turning on the light) for 4 h either to dark (0 µE, 22 °C) or low light (100-130 µE, 22 °C) conditions and subsequently harvested.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Christian Lindermayr

PROVIDER: E-MTAB-9299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq of Arabidopsis thaliana leaves for three genotypes (Col-0 wild type, gsnor1-3, axe1-5/hda6) in low light and dark conditions

Project description:Plants were grown for 4 weeks under short day (10 h light/14 h dark and 20 °C/16 °C, respectively). The relative humidity during the day and night was 50 %. Then, plants were transferred at 11 am (5 h after turning on the light) for 4 h either to dark (D, 0 µE, 22 °C) or low light (LL, 100-130 µE, 22 °C) conditions and subsequently harvested.

2021-05-28 | E-MTAB-9301 | biostudies-arrayexpress

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Project description:Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, showing the presence of four introns that excluded a potential bacterial contamination. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated for early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in N. benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.

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Project description:We identified a small zinc finger protein, MBS, as a new mediator of singlet oxygen responses in Chlamydomonas and Arabidopsis. MBS is required for induction of singlet oxygen-dependent gene expression and, upon oxidative stress, accumulates in distinct granules in the cytosol of Arabidopsis cells. First, we recorded changes in light stress-regulated gene expression profiles after genetically perturbing MBS function by isolating mutants for the two MBS genes (MBS1 and MBS2) and by overexpression of MBS1 in Arabidopsis thaliana. Then, these light stress-related gene expression profiles were analyzed with respect to genes specifically responding to singlet oxygen and hydrogen peroxide/superoxide. The results indicated that MBS inactivation leads to an impaired response to singlet oxygen signaling under light stress. Knock-out or knock-down of the MBS1 and MBS2 genes and overexpression of MBS1 in Arabidopsis thaliana were compared with the wild type and the flu mutant as controls under light stress. Arabidopsis seedlings of mbs1-1, RNAi-MBS2/mbs1-1, 35S:MBS1, the wild type and the flu mutant were harvested at 0 h and 3 h of light stress (HL, 1000 µE m−2 s−1). Total RNA was extracted and cDNA was hybridized to Affymetrix ATH1 microarrays in three biological replicates.

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Project description:Series of 6 repetitions of hybridization of treatment (HL_1h) and control (0h) each. Comparison of plants exposed to light stress at 1000 µE for 1 h versus plants grown under normal light conditions of 80 µE. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample

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