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Microarray of MC38-R tumors following intratumoral injection of IL-12 mRNA or control mRNA

ABSTRACT: We designed a novel intratumoral (IT) IL-12 mRNA therapy to promote local IL-12 tumor production whilst mitigating systemic effects. A single IT dose of mouse (m)IL-12 mRNA induced IFNγ and CD8+ T cell-dependent tumor regression in multiple syngeneic mouse models including MC38-R, and animals with a complete response demonstrated immunity to re-challenge. In order to further investigate the impact of mIL-12 mRNA on the MC38-R TME, we performed tumor transcriptomic evaluation 24h and 7 days after a single IT dose of 0.5 micrograms IL-12 or control mRNA. mRNA was isolated from snap-frozen syngeneic tumors using an RNeasy mini kit (Qiagen) and quantified on the Affymetrix mouse 430 2.0 microarray.

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Morehouse

PROVIDER: E-MTAB-9316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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