Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis of MDCK parental cells (ATCC vs ECCAC) in response to CAV2 infection.

ABSTRACT: Phenotypic variability in cultured mammalian cell lines has long been recognized and known to be driven by passaging and culture conditions. Yet, the effect these variations have on the production of gene therapy viral vectors has been largely overlooked. In this work we evaluated the impact of using MDCK parental cells from ATCC or ECACC for the production of canine adenoviral vectors type 2 (CAV-2) in adherent and suspension cultures. Additionally, we conducted whole-genome transcriptome analysis to further explore the differences between cells. Our results indicate that ECACC parental cells are a less heterogeneous population, relatively to ATCC’s, more difficult to adapt to suspension and serum-free culture conditions, but more permissive to CAV-2 replication progression enabling higher yields. Transcriptome data indicates that this increased permissiveness is due to a general down-regulation of biological networks of innate immunity in ECACC cells.

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Ana Filipa Rodrigues

PROVIDER: E-MTAB-9379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of MDCK parental cells (ATCC vs ECCAC) in response to CAV2 infection.

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