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Single-cell transcriptome of cerebral organoids with and without methanol fixation

ABSTRACT: To study how methanol fixation affects single-cell transcriptomic measurement, two cerebral organoids were dissociated. Cell suspension of each organoid was split into two aliquots. Methanol fixation was applied to one of the two aliquots. Single-cell RNA-seq with 10x Genomics was applied to the two aliquots separately.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Zhisong He

PROVIDER: E-MTAB-9473 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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