Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

DNA sequencing to validate random deletions in Mycoplasma pneumoniae


ABSTRACT: We present LoxTnSeq, a new methodology to generate and catalogue libraries of genome reduction mutants. LoxTnSeq combines random integration of Lox sites by transposon mutagenesis, and the generation of mutants via cre recombinase, catalogued via deep-sequencing. When LoxTnSeq was applied to the naturally genome reduced bacterium Mycoplasma pneumoniae, we obtained a mutant pool containing 285 unique deletions. These deletions spanned from >50 bp to 28 Kb, which represent 21% of the total genome. LoxTnSeq also highlighted large regions of non-essential genes that could be removed simultaneously, and other similar regions that could not, providing a guide for future genome reductions.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mycoplasma pneumoniae

SUBMITTER: Maria Lluch-Senar 

PROVIDER: E-MTAB-9582 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2021-08-18 | E-MTAB-9590 | biostudies-arrayexpress
2022-04-06 | E-MTAB-11600 | biostudies-arrayexpress
2023-07-31 | E-MTAB-10380 | biostudies-arrayexpress
2019-07-31 | E-MTAB-6124 | biostudies-arrayexpress
2012-03-01 | E-GEOD-31880 | biostudies-arrayexpress
2012-03-01 | E-GEOD-31881 | biostudies-arrayexpress
2012-03-01 | E-GEOD-31882 | biostudies-arrayexpress
2010-05-17 | E-GEOD-15786 | biostudies-arrayexpress
2020-08-28 | E-MTAB-8918 | biostudies-arrayexpress
2018-09-03 | PXD009557 | Pride