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MicroRNA profiling of human breast cancer cell lines using five miRNA microarray platforms

ABSTRACT: Analysis of hybridisation performance and utility in identifying differentially expressed miRNAs of six miRNA microarray platforms using three biological samples in quadruplicate.

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Anna Git

PROVIDER: E-MTAB-96 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression.

Git Anna A Dvinge Heidi H Salmon-Divon Mali M Osborne Michelle M Kutter Claudia C Hadfield James J Bertone Paul P Caldas Carlos C

RNA (New York, N.Y.) 20100401 5

RNA abundance and DNA copy number are routinely measured in high-throughput using microarray and next-generation sequencing (NGS) technologies, and the attributes of different platforms have been extensively analyzed. Recently, the application of both microarrays and NGS has expanded to include microRNAs (miRNAs), but the relative performance of these methods has not been rigorously characterized. We analyzed three biological samples across six miRNA microarray platforms and compared their hybri ...[more]

PMID: 20360395

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