Illumina sequencing analysis of mutagenicity of EvoluGene

ABSTRACT: We created a mutator protein, Evolugene with very fast in vivo mutation rate and gene specificity in E. coli. To comprehensively analyze the specificity and mutagenicity, we sequenced ~3.3 kb DNA around the target gene from cells taken at cycle 27 by Illumina sequencing.

INSTRUMENT(S): Illumina HiSeq 2500, TruSeq Nano DNA Kit, Cosmogenetech

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Seokhee Kim 

PROVIDER: E-MTAB-9677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress