Project description:The transcriptome of Ctrl and Vitamin A-deficient longterm hematopoietic stem cells (LT-HSC) and multipotant progenitors (MPP3/4) was assessed by RNAseq.
Project description:The distribution of H3K4- and H3K27me3 on the chromatin of mouse longterm hematopoietic stem cells and multipotent progenitor cells has been profiled by ChIP-seq.
Project description:The transcriptome of WT and Rarb KO hematopoietic stem cells (HSC) after 24h in vitro culture upon retinoid treatment was assessed by RNAseq.
Project description:The transcriptome of Ctrl and Cyp26b1KO hematopoietic stem cells (HSC) after 24h in vitro culture upon retinoid treatment was assessed by RNAseq.
Project description:4 months old Scl-tta; H2B-GFP mice where chased with Doxycyclin for 18 months from and then sacrificed at 22 months of age. GFP+ and GFP- cells, or dormant and active HSCs were then sorted and sequenced.
Project description:To study the role of the receptor Neo1 in Hematopoietic stem cells (HSC) we transplanted Neo1 deficient bone marrow and wildtype controls on a CD45.2 background into lethally irradiated CD45.1 recipients. Either 4 or 15 months after transplantation we isolated HSC (Lineage -, Sca-1+, c-Kit+, Cd150+, CD48-, CD34-, CD45.2+) to identify molecular differences due to Neo1 deficiency and found a loss of HSC quiescence and signatures associated with decreased stem cell function.