Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Bulk RNA Barcoding and sequencing (BRB-seq) of Mock, SARS-CoV, and SARS-CoV-2 infected human airway epithelial cell cultures at 33°C and 37°C

ABSTRACT: For the assessment of host response dynamics to SARS-CoV and SARS-CoV-2 infections in human airway epithelial cells at ambient temperature corresponding to the upper or lower respiratory tract. We performed a temporal transcriptome analysis on human airway epithelial cell (hAEC) cultures infected with SARS-CoV and SARS-CoV-2, as well as uninfected hAEC cultures, incubated either at 33°C or 37°C. hAEC cultures were harvested at 24, 48 72, 96 hpi and processed for Bulk RNA Barcoding and sequencing (BRB-seq), which allows a rapid and sensitive genome-wide transcriptomic analysis in a highly multiplexed manner. Transcriptome data was obtained from a total of 7 biological donors for pairwise comparisons of SARS-CoV or SARS-CoV-2 virus-infected to unexposed hAEC cultures at respective time points and temperatures.

ORGANISM(S): Homo sapiens

SUBMITTER: Ronald Dijkman

PROVIDER: E-MTAB-9781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium.

V'kovski Philip P Gultom Mitra M Kelly Jenna N JN Steiner Silvio S Russeil Julie J Mangeat Bastien B Cora Elisa E Pezoldt Joern J Holwerda Melle M Kratzel Annika A Laloli Laura L Wider Manon M Portmann Jasmine J Tran Thao T Ebert Nadine N Stalder Hanspeter H Hartmann Rune R Gardeux Vincent V Alpern Daniel D Deplancke Bart B Thiel Volker V Dijkman Ronald R

PLoS biology 20210329 3

Since its emergence in December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread globally and become a major public health burden. Despite its close phylogenetic relationship to SARS-CoV, SARS-CoV-2 exhibits increased human-to-human transmission dynamics, likely due to efficient early replication in the upper respiratory epithelium of infected individuals. Since different temperatures encountered in the human upper and lower respiratory tract (33°C and 37°C, respecti ...[more]

PMID: 33780434

Dataset Information

Bulk RNA Barcoding and sequencing (BRB-seq) of Mock, SARS-CoV, and SARS-CoV-2 infected human airway epithelial cell cultures at 33°C and 37°C

Publications

Disparate temperature-dependent virus-host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium.

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