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Transcription profiling by array of Nab2 depletion effect on yeast mRNA

ABSTRACT: Transcription profiling was performed on the time course of Nab2 protein depletion in THC-Nab2 strain at 0h, 7h, 12h in biological triplicates to access its affect on the composition and structure of mRNA in budding yeast. For the protein depletion experiment THC-NAB2 (THJ collection Y2484, THC-NAB2:KAN tTA:URA, Open Biosystems) strain was used, where expression of the NAB2 gene is under control of a tetracyclin-repressible promoter.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Charles Girardot

PROVIDER: E-MTAB-981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins.

Schmid Manfred M Poulsen Mathias Bach MB Olszewski Pawel P Pelechano Vicent V Saguez Cyril C Gupta Ishaan I Steinmetz Lars M LM Moore Claire C Jensen Torben Heick TH

Molecular cell 20120607 2

Poly(A) (pA) tail binding proteins (PABPs) control mRNA polyadenylation, stability, and translation. In a purified system, S. cerevisiae PABPs, Pab1p and Nab2p, are individually sufficient to provide normal pA tail length. However, it is unknown how this occurs in more complex environments. Here we find that the nuclear exosome subunit Rrp6p counteracts the in vitro and in vivo extension of mature pA tails by the noncanonical pA polymerase Trf4p. Moreover, PABP loading onto nascent pA tails is c ...[more]

PMID: 22683267

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