Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

97 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of control and GPRC5C-Overexpression (GPRC5C-OE) in human mobilized peripheral blood (mPB)

ABSTRACT: The human transcriptome of lentiviral GPR-OE and control was assessed by RNAseq.

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Homo sapiens

SUBMITTER: Mari Carmen Romero-Mulero

PROVIDER: E-MTAB-9874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of GPRC5C-Knockdown (GPRC5C-KD) and control LT-HSCs from human bone marrow

Project description:The transcriptome of shRenilla and shGPRC5C in long term hematopoietic stem cells (LT-HSC) from human bone marrow was assessed by RNAseq.

2022-04-06 | E-MTAB-11427 | biostudies-arrayexpress

RNA sequencing of GPRC5C-Knockdown (GPRC5C-KD) and control CD34+ human bone marrow

Project description:The transcriptome of shGPR and shRenilla in human CD34+ bone marrow was assessed by RNAseq.

2022-04-06 | E-MTAB-9863 | biostudies-arrayexpress

RNA Sequencing of LT-HSCs and ST-HSCs from healthy adult human Bone Marrow

Project description:The transcriptome of LT-HSC (CD34+CD38-CD45RA+CD90+CD49f+) and ST-HSC (CD34+CD38-CD45RA+CD90-CD49f-) from healthy adult human Bone Marrow Cells were assessed by RNA-seq.

2022-04-06 | E-MTAB-9892 | biostudies-arrayexpress

RNA Sequencing of Gprc5c-Knockout (Gprc5c-KO) and wildtype (WT) control mice HSCs.

Project description:The transcriptome of Gprc5c-KO and WT control mice HSCs were assessed by RNA-seq.

2022-04-06 | E-MTAB-9862 | biostudies-arrayexpress

Single Cell RNA Sequencing of human bone marrow LT-HSCs.

Project description:10x single cell sequencing of LT-HSCs (CD34+CD38-CD90+CD45RA-CD49f+) from human bone marrow.

2022-04-06 | E-MTAB-9922 | biostudies-arrayexpress

Gene expression profiles of AML derived stem cells: similarity to hematopoietic stem cells

Project description:Tumors contain a fraction of cancer stem cells that maintain the propagation of the disease. The CD34‏CD38_ cells, isolated from acute myeloid leukemia (AML), were shown to be enriched leukemic stem cells (LSC). We isolated the CD34‏CD38_ cell fraction from AML and compared their gene expression profiles to the CD34‏CD38‏ cell fraction, using microarrays. We found 409 genes that were at least twofold over- or underexpressed between the two cell populations. These include underexpression of DNA repair, signal transduction and cell cycle genes, consistent with the relative quiescence of stem cells, and chromosomal aberrations and mutations of leukemic cells. Comparison of the LSC expression data to that of normal hematopoietic stem cells (HSC) revealed that 34% of the modulated genes are shared by both LSC and HSC, supporting the suggestion that the LSC originated within the HSC progenitors. We focused on the Notch pathway since Jagged-2, a Notch ligand was found to be overexpressed in the LSC samples. We show that DAPT, an inhibitor of gamma-secretase, a protease that is involved in Jagged and Notch signaling, inhibits LSC growth in colony formation assays. Identification of additional genes that regulate LSC self-renewal may provide new targets for therapy. Microarrays were used to compare the gene expression patterns between AML CD34+CD38- cells and AML CD34+CD38+

2011-12-01 | E-GEOD-34044 | biostudies-arrayexpress

Transcription profiling of human gene expression profiles of CD34+/CD38- cells vs AFT024

Project description:It is widely accepted that hematopoietic progenitor cells (HPC) are tightly associated with discrete niches within the bone marrow. This molecular environment supports and regulates their self renewal and differentiation. AFT024 is a cell line derived from murine fetal liver that has been demonstrated to maintain human hematopoietic progenitors in an undifferentiated state in vitro. While various functional and genomic studies of this and other stromal layers have already been reported, the influence of the cellular microenvironment on the gene expression of HPC has not yet been systematically analyzed.<br> <br> The CD34+/CD38- fraction of human umbilical cord blood was parted and either cultivated on AFT024 [kindly provided by I. Lemischka] or without stromal feeder layer for 16h, 20h, 48h or 72h. Both fractions were then harvested and separated by vigorous pipetting and FACS sorted again to separate the HPC from AFT024. Global gene expression profiles were determined using a novel Human Genome cDNA Microarray of over 51,145 ESTs of the UnigenSet-RZPD3. In analogy, we have compared the gene expression profiles of CD34+/CD38- cells versus AFT024 to exclude that differential gene expression resulted from contaminating feeder layer cells.

2005-06-13 | E-EMBL-3 | biostudies-arrayexpress

miRNA profiling of human acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment.

Project description:The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38- fraction.

2010-02-19 | E-TABM-909 | biostudies-arrayexpress

Methylation arrays (MethylationEPIC) reveal specific DNA hypermethylation of T cells during human hematopoietic differentiation

Project description:Epigenetic regulation plays an important role in cellular development and differentiation. A detailed map of the DNA methylation dynamics that occur during cell differentiation would contribute to decipher the molecular networks governing cell fate commitment. We used the most recent Illumina MethylationEPIC Beadchip platform to describe the genome-wide DNA methylation changes observed throughout hematopoietic maturation by analyzing multiple hematopoietic cell types at different developmental stages. We identified a plethora of DNA methylation changes that occur during human hematopoietic differentiation. Interestingly, we observed that T lymphocytes display a substantial enhancement of de novo CpG hypermethylation as compared to other hematopoietic cell populations.

2018-06-08 | E-MTAB-6315 | biostudies-arrayexpress

Transcription profiling of CD34+CD38- vs CD34+CD38+ fractions of human umbilical cord blood and comparison of slow-dividing fraction vs the fast dividing fraction of CD34+/CD38- population

Project description:Comparison of the CD34+CD38- versus CD34+CD38+ fractions of human umbilical cord blood and comparison of the slow-dividing fraction versus the fast dividing fraction of the CD34+/CD38- population.

2005-09-08 | E-EMBL-1 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data